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Cells were stained simultaneously with 20 µM propidium iodide, which is excluded from viable cells but exhibits a red fluorescence following a loss of membrane integrity, and 4.5 µM Hoechst 33342 (Molecular Probes, Eugene, OR), which stains all nuclei and yields blue fluorescence to UV illumination, to count the total number of cells.
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(If this by itself were to count as illumination, then all theists would be committed to the theory of divine illumination).
To ensure that participants concentrated on the video, they were also told to count changes in global illumination that occurred during the video.
"Majorities have to count.
"Too many to count.
To count cattle?
Images were taken under bright-field illumination using a Nikon Eclipse 80i compound fluorescent microscope at 20× magnification and analyzed using the open-source ImageJ software (http://rsb.info.nih.gov/ij/) to count the number of stomata per field of view.
Learn to count music.
Try to count.
Learn how to count.
Learn to count.
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CEO of Professional Science Editing for Scientists @ prosciediting.com