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Signal collection is based on a custom-made 64-element focused transducer array (Imasonic SaS, Voray, France) covering a solid angle of 172° around the imaged object, while the detection plane coincided with the center of the illumination ring.
The 50 μm × 50 μm detector location is positioned in the center of the illumination ring.
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Beside the main advantages of SLOT compared to OPT namely homogeneous illumination, prevention of ring artifacts and a high collection efficiency [ 19] the SLOT setup additionally allows the image formation by detection of scattered laser light.
The SDF camera is equipped with a 5x magnifying objective lens system-containing probe, imaging the red blood cells (RBCs) in the tissue-embedded microcirculation using green pulsed LED ring illumination [ 14, 34].
Recently, we introduced scanning laser optical tomography (SLOT) [ 19] which can be seen as an improvement of optical projection tomography (OPT) [ 20] in terms of homogeneous illumination, prevention of ring artifacts and increase of photon collection efficiency.
UV illumination generates the ring-opened merocyanine form (Mc), which exhibits a much higher p Ka (2).
An interesting recent example utilized the light-induced ring-opening of spiropyrans under ultraviolet light and their ring closure under visible light illumination.
The calculation model of light illumination properties of LED ring array light source are set up and analyzed, light illumination calculation functions of ring source are derived; based on design characteristics of ring source, illumination model and calculation principles of uniform light source are analyzed, the derivation process is established in detail.
The optical axicon with an angle ζ 3 generated a ring shaped illumination pattern on the optical absorber.
The excitation source is a ring light used for epi-illumination, mounted 12 cm above the mice.
We have developed a portable ring-light illumination module used to acquire photometric image stacks of holograms with mobile devices.
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