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However, the onset of VTP induced a prompt increase in BOLD contrast signals exclusively in the tumor area, which continued to rise throughout the illumination phase and peaked at 30% above baseline values (Figure 7A, n = 6).
Top row: each structured illumination phase is shown.
During the course of the illumination phase of the experiment, cellular autofluorescence levels were measured and provide insight into the complexity of the photobleaching and photodamage mechanisms as a function of femtosecond laser power.
A typical response to blue or red light included a burst of activity at light onset lasting approximately 30 s, followed by a return to near baseline activity levels, a gradual increase in activity that reached a plateau for the remainder of the illumination phase, and a burst of activity when the lights were turned off.
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In fact, an increase in dissolved oxygen or pH creates unfavorable conditions for MFC operation and would normally be expected to reduce MFC voltage during the illumination-phase.
In contrast to the rapid rise of cell voltage observed in direct response to illumination, both dissolved oxygen and pH displayed more gradual increase during 12 h illumination-phase.
Here, overnight illumination could be shown to reduce homecage activity during the dark phase (p<0.01) while light phase activity remained unchanged.
Light-adapted mice were housed under room illumination (∼750 lux) and the eyes were collected at the mid-point of the light phase.
In the initial nominal state for LCP feed illumination, the phase in the left side aperture region is leading compared with the phase in the right side aperture region, which causes the radiated RCP beam to squint toward the left in xz plane and produces a positive squint angle.
ULTI cells subcultured onto plastic dishes were examined with Köhler illumination by phase contrast microscopy on a Zeiss Axiovert 200M inverted microscope using the 20x objective.
Protoporphyrin IX-specific fluorescence in the glioma cells was captured by fluorescence microscopy (BX 50, Olympus) and a digital camera (DP70, Olympus) under the same exposure time of the illumination source: phase-contrast images, 1/60 s, and PpIX molecular images, 20 s.
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