Sentence examples for illumination objectives from inspiring English sources
Light sheets were generated with cylindrical lenses (f=50 mm) and projected with telescopes and the illumination objectives onto the focal plane of the detection lens.
The sample chamber between the illumination objective and detection objective 1 is drawn in more detail on the right side of the schematic showing the position of the scattering layer and of the glass capillary containing the sample.
The high NA of the illumination objective resulted in a strong convergence of the focused beam and a corresponding short working distance of the illumination objective.
As illumination objective we used a plan apochromat 10×, NA 0.28 long working distance objective lens (Mitutoyo, Japan).
A special long working distance illumination objective (10×; NA = 0.28; working distance, 33.5 mm; Mitutoyo, Japan) met all construction conditions.
The light sheet had to be created within the working distance of 280 µm of the detection objective and was produced by a dedicated illumination objective lens with a high NA in order to achieve a thin light sheet with an axial extension of only 3 µm full-width-at-half-maximum (FWHM).
Z.1 light sheet fluorescence microscope system was equipped with a Carl Zeiss W Plan-apochromat 20x/1.0 detection objective and a Carl Zeiss LSFM 10x/0.2 illumination objective.
The forward generated CARS signal is collected by a 40X/0.8NA water immersion objective (Olympus, LUMPlanFl/IR), while the epi-detected signals returns through the illumination objective.
The laser is incident vertically onto a resonant mirror (RM) which is conjugated to the image plane of the illumination objective (Nikon CFI Plan Fluor 10 ×W).
Because the infinity-correction range of the illumination objective is limited, the light sheet abruptly expanded in z-direction at TAG modulation intensity > 35% (Supplementary information, Figure S1E).
A galvo scanning mirror (GSM) was used to scan the laser beam vertically to the axis of the illumination objective (y-direction).
