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We adopted a simplified version of LIPVCD technique, in which a single illumination light source and a laboratory oscilloscope could be conveniently used for the measurements.
Among them, the illumination light source adopts the flexible circuit board annular 850 nm LED light source, light through the Placido disc that can make the eye table brightness and illumination uniformity, it is conducive to obtain a clear eye plaque Placido annular image using the image acquisition system.
Light microscopy was performed on an inverted microscope (Axiovert 100TV; Carl Zeiss, Jena, Germany) using standard epifluorescence illumination (light source HXP120, Zeiss) and 63×/NA1.4 or 100×/NA1.4 plan-apochromatic objectives.
However, since the diffuse illumination light source for color measurement cannot be turned on and off frequently, the gloss must be photographed with diffuse illumination at all times.
The system used a super luminescent diode as the illumination light source, with a central wavelength of 1300 nm and a bandwidth of 80 nm that provided a ~10 µm axial resolution in air.
For the imaging of eGFP, an illumination light source passes through a band-pass filter with a central wavelength of 475 nm to excite the protein, and a band-pass detection filter with a central wavelength of 525 nm selectively captures the emission light of the excited eGFP.
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Arrangements with three sets of different type fibers are tested in this study and output powers are monitored with the white lamp or red LED as illumination light sources.
Unfortunately, although the Lee No. 16 filter, has a (slightly) larger acquisition window (approx. 475 700 nm) and higher transmittance, in this case, the higher transparency partly overlaps with the Blue LED emission spectrum and allows too much of the reflected light through from the illumination source (as is apparent from the 99% tile of the Spectralon® reference scale in these images).
Compiling more evidence on both evidence based decision making and decision based evidence making will turn the lamp post that serves as a prop into an illumination source that lights up the path of choice.
A 100-W Xe arc lamp was used as the illumination source for light-assisted gas-sensing tests.
As for possible reflections from the surrounding surfaces, we assume these are negligible in comparison with the light from the illumination sources.
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