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The optical field in a nearby region of an illuminated sample consists of both propagating and evanescent field components.
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Even under optimal conditions for illumination, several intermediate states could coexist in the illuminated samples.
Samples with RB were kept in the dark (dark control) for 10 and 30 min and illuminated samples without RB (light control) served as controls.
'Surface colors' are the colors of illuminated samples seen under conditions in which it is possible for the viewer to distinguish the color of the surface from that of the ambient light.
The illuminated samples were immersed in 3 5 ml of cellulase solution at 37 °C overnight.
Indeed, in our system the senescence program leading to cell death was initiated in illuminated samples.
We also investigated which genes were significantly expressed in continuously illuminated samples under each condition.
The samples incubated in the dark in the presence of DBMIB lay in a position between the illuminated samples and the dark-incubated samples.
In the presence of DCMU or DBMIB, the plots of PC1 scores were closer to those of dark-acclimated samples than those of illuminated samples.
A comparison of the results obtained for darkened and illuminated samples shows that PAR is a key factor in the induction of senescence-associated genes by UV-B.
Figure 1d shows the results of the PCA and indicates that the difference in transcription profiles between the illuminated samples and dark-acclimated samples is represented primarily in PC1.
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CEO of Professional Science Editing for Scientists @ prosciediting.com