Sentence examples similar to illegitimate clone from inspiring English sources
Yet, we wanted to further understand why a large proportion of recombined clones had undergone an illegitimate recombination.
When N6 carrying the empty vector pILL2150 was transformed with either of the two suicide plasmids, we obtained either no kanamycin resistant clones or a couple of clones that were either non viable or had undergone illegitimate recombination (Fig. 1).
Still, screening for the right clone can be tedious because the high incidence of illegitimate assembly results in a relatively large proportion of missing or shuffled DNA elements.
A high number of clones was obtained and sequence analysis confirmed the illegitimate recombination between attP4R and attP5, resulting in a loss of the ccdB and chloramphenicol resistance genes.
These illegitimate recombinations were considered as acquired CNVs [5], characteristic of the malignant clone.
Most of the clones from populations selected with MTX and GCV and failing to express GFP contained both correct and illegitimate insertions (data not shown).
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