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HL-1 cells were plated in 2-well Lab-Tek® II chamber slides and treated for 18 h.
Cells used in time-lapse microscopy were grown on an eight-well Lab Tek II chamber glass slide (Nunc, VWR International, Leuven, Belgium).
Cells were grown on Lab-Tek II chamber slide (Nunc), rinsed in PBS, fixed in 4% paraformaldehyde for 20 min, and rinsed again in PBS.
MC57 and BalbCl7 cells (200,000 and 80,000 cells/well respectively) were cultured on Lab-Tek II chamber polylysine slides (Nalge Nunc Naperville, IL) and CPMV-FITC was added to the media.
Cells were grown on Lab-Tek II chamber slide (Nalge Nunc Int., Naperville, IL), rinsed in PBS, fixed in 4% paraformaldehyde for 20 min, and rinsed again in PBS.
HEK 293 cells were cultured on Lab-Tek II chamber glass slides (Nalge Nunc, Naperville, IL), serum-starved for 24 h, and treated with 200 ng/mL Wnt3a for different lengths of time.
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A total of 4000 LNCaP cells were plated on each well of an eight-well Lab-Tek II® chamber slide (Nalge Nunc International, Naperville, IL, USA).
For fluorescent staining of intracellular sporozoites, HepG2 cells [ 59] were grown in Lab-Tek™ II Chamber Slides™ (Nunc, 154534) overnight followed by infection with 30,000 sporozoites per chamber for at least 2 hrs.
Nunc™ Lab-Tek™ II Chamber Slide™ Systems were analyzed by an inverted confocal microscope (Leica TCS SP5 II) using an objective with ×20 magnification.
To determine BODIPY-BBN uptake in vitro, PC-3 cells (1.0 × 104 per well) were seeded in a two-well chamber slide (Nunc™ Lab-Tek™ II Chamber Slide™ System) containing growth media (1 mL) and incubated at 37 °C for 24 h.
HEK293T cells were cultured on chamber slides (Lab-Tek II Chamber Slide w/Cover RS Glass Slide Sterile, Nunc) and transfected with the vectors pcDNA3X HA-sik1 (1 249) and pcDNA3X HA-rSik1 using the PolyFect Transfection Reagent (Qiagen).
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