If the targeted animal stands its ground, the wolves either ignore it, or try to intimidate it into running.
An important design aspect of our system is that the targeted vectors are ablated for CAR binding, thereby eliminating the possibility of ectopic vector localization in vivo to CAR-expressing tissues if the targeted complex is disrupted.
If the targeted H. sapiens proteins are mostly located in cellular compartments having a close relationship with pathogen infection then the predicted results are more convincing.
If the targeted heart-rate reduction was not obtained, a second 1-min infusion and 10-min continuous intravenous infusions were given at an increased dose.
If the targeted H. sapiens proteins are mostly located in cellular compartments having a close relationship with pathogen infection or known interactions with host cells, then we can be more certain about the quality of our results.
If the targeted heart-rate reduction (≥20 % reduction from the baseline heart rate and a heart rate of <100 beats/min) was obtained after administration of the initial dose, the dose was not increased.
If the targeted H. sapiens proteins are located in cellular compartments that are very relevant to the pathogen's infection or are very likely to be involved in interactions with the pathogen, then the result supports the host-pathogen predictions.
Obvious drawback of such design is that patients mostly do not undergo kidney biopsy at study entry (especially in case of diabetic nephropathy and nephrosclerosis) and that there is no knowledge if the targeted disease mechanism is even active.
First, the expression of PDGFR α, NG2, Olig2, or Plp are not exclusive in NG2+ cells, making NG2+ cell-fate mapping less straightforward, and if the targeted genes were not specific to NG2+ cells at the time of t.i., the integrity of the genetic cell-fate tracing experiments would be compromised.
To further explore if the targeted Fmp-IO-Pc 4 NPs have any advantage in efficacy over the nontargeted IO-Pc 4 NPs, we administered the mice IO-Pc 4 and Fmp-IO-Pc 4 at a low dose of 0.06 mg/kg Pc 4 equivalent.
If the targeted time comes before you feel the need to go, still go anyway to empty your bladder.
