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To demonstrate the feasibility of our methodology, we showed that is it possible to obtain accurate and reliable P- and S-wave ultrasonic velocities measurements and the Thomsen parameters (ε and γ) related to them, even if the sample presents a high fracture density, due to the high quality of the acquired waveform, expressed in a high signal-to-noise ratio.
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If the sample presented small spots on the surface at the end of the process, as observed under an optical microscope, the polishing steps was repeated.
However, if the samples presented ties, we applied a variation of the Wilcoxon test, the Asymptotic paired, two-sided Wilcoxon (Signed Rank) (Kohl 2015), suitable to treat ties, with significance level α = 0.05.
Finally, one potential situation in which NSA could fail is if all the samples present an aberration in the same locus.
24 A trial was classified as meeting the sample size requirement if the sample size calculation was presented and clearly accounted for clustering (such as by using the intracluster correlation, coefficient of variation, or cluster level summary statistics).
The RNA quantitation method we employ, namely the binding of the fluorescent dye RiboGreen, provides an overall assessment of the amount of RNA present, but cannot provide quantitative information regarding the amount of each fluid present if the sample contains more than one body fluid.
This positive correlation is also present if the sample is broken down into distinct theistic views (creationist and non-creationist viewpoints).
Highly specific genes tend to be expressed in a small subset of the cells that form an organ and thus have a high probability of not having any gene tags and not being present if the sample is relatively small.
If MEE was not present or if the sample was culture and/or PCR negative, probes for the most prevalent organisms were used (S. pneumoniae, M. catarrhalis or H. influenzae).
Daphnia and free ephippia were counted using a stereomicroscope 10-200 × magnification) whereby we also recorded the presence of other aquatic animals in the sample, if present.
Peritoneal fluid, if already present, will be sampled prior to any manipulation of the tumour and if the sample gives <50 mls, 100mls of normal saline will be instilled around the primary tumour and 50mls recuperated from the epigastric/left hypochondriac regions after a delay of 2 minutes without tumour manipulation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com