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It is possible that the shorter phosphine-FLAG reagents, even if reacted with the site deep in the binding pocket, cannot be efficiently detected because the epitope is not fully exposed to the antibody.
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Feeling bad at least feels arguable, like if I reacted with, "Boo!
Republican presidential candidates, who have universally pledged to overturn Obama's executive orders if elected, reacted with swift outrage to the president's announcement.
What if we reacted with vulnerability rather than defensiveness?
Or if Maria Shriver reacted with surprise and shock at the recycled revelations rather than support for her movie star husband.
The sample was also considered positive for an undetermined flavivirus if it reacted with WNV.sE but not with any of the rEDIIIs.
Sera were defined as being positive if they reacted with one or both of the MUC1a and MUC1b glycopeptides that carried the same glycan, taking the maximum of the two values.
Instead, the final product is trans-Pt(GS 2(NH3)2 (at −3215 ppm); the same product appears if GSH is reacted with trans-PtCl2(NH3)2.
If the antibody reacted with the chip, they knew the blood sample contained A toxin.
Isolates were tentatively identified as belonging to the genus Ralstonia if they 1) reacted with primer pair RHG-F/RHG-R (specific for Burkholderia and Ralstonia spp).
If a protein reacted with a serum antibody called IgE which plays a role in nearly all allergies it was flagged as an allergen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com