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If no database is available, patient records and pharmacy and laboratory registers may provide valuable information but if these are not electronic, data collection can be very time consuming.
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It was noted that no database was available for algae.
However, it is difficult to estimate the precise numbers of repetitions even if the complete database is available.
If your organism of interest is not included in the list here, please check the corresponding websites, or contact the proteomics facility to see if a FASTA format database is available.
Further, because the analysis is statistics-based, the methodology can be expanded to higher eukaryotes if a morphologic phenotype database is available.
The methodology proposed in the present document can be reproduced in other countries if a population census database is available.
Therefore, even if a large EST database is available for a target species, ESTs have limitations as a material for development of SSR markers for specific genes.
In case of pig (Sus scrofa), the genome database is available, but no GBGT1 genes have been annotated.
The database is available at http://www.bgs.ac.uk/vogripa with no restrictions on access.
tBLASTN and BLASTP (if the protein database were available) or BLAT searches were performed using the HMG domain of the well-defined Sox group B member SOX1 of Homo sapiens as the query with the current assemblies and predicted proteins in the genomes of the examined species to identify and/or retrieve the Sox group B member sequences.
In the case of maritime pine, no sequence database was available to test for sequence redundancy.
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