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The IDS assay was performed as described previously (35): briefly, 50 µg total protein extract was incubated with 20 µl of the fluorogenic substrate, 4-methylumbelliferyl-α-iduronide-2-sulfate (Muscerdam Substrates), for 4 h at 37°C.
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In this study, we developed a new SNP typing method, named the amplified-product length polymorphism (APLP) 48-ID assay, for genotyping of 47 autosomal SNPs and two X and Y chromosomal markers for sex typing.
With the PLEX-ID assay, seven PHPs were called that were primarily not identified with STS.
The PLEX-ID assay targeted 1049 bases of the CR covering all three hypervariable segments.
Table S3 PHPs detected by the STS assay, but not or only partially detected with the PLEX-ID assay.
Table S4 PHPs detected by the PLEX-ID assay, but not detected with the STS assay at first call.
Four samples showing LHP in the C-stretch were concordant between STS and PLEX-ID assays, two additional samples were detected by STS and one additional sample by the PLEX-ID assay.
Fig. S3 PHPs not (B) or only partially detected by the PLEX-ID assay (A, C F).
The following are the supplementary data to this article: Fig. S2 BC distribution and variation across the PLEX-ID assay.
Rows in grey mark PHP positions that could be detected in the PLEX-ID assay at second call.
Dominant variants detected by the STS assay are labeled STSBC whereas dominant variants called by the PLEX-ID assay are labeled PLEXIDBC.
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