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Therefore, the HID-Ion AmpliSeq™ Identity Panel and Ion PGM™ system provide a sensitive and accurate forensic SNP genotyping assay.
Except for 18 loci with He <0.4, most of the A-SNPs in the HID Identity Panel presented high polymorphisms.
The sequence coverage obtained was extensive for the bulk of SNPs targeted by the HID-Ion AmpliSeq™ Identity Panel.
The HID-Ion AmpliSeq™ Identity Panel (the HID Identity Panel) is designed to detect 124-plex single nucleotide polymorphisms (SNPs) with next generation sequencing (NGS) technology on the Ion Torrent PGM™ platform, including 90 individual identification SNPs (IISNPs) on autosomal chromosomes and 34 lineage informative SNPs (LISNPs) on Y chromosome.
To support the implementation of SNPs in forensic DNA analysis, NIST has examined the HID-Ion AmpliSeq Identity Panel and the HID-Ion AmpliSeq Ancestry Panel for the Ion Torrent Personal Genome Machine (PGM).
In this study, we evaluated performance for the HID Identity Panel to provide a reference for NGS-SNP application, focusing on locus strand balance, locus coverage balance, heterozygote balance, and background signals.
Similar(49)
The identity of panel members was known by the moderator, but not to other panel members.
Cells masks are colored by cell identity (left panel); GFP intensity with B. thai cell positions outlined in white dashed lines (right panel).
The identity of the panel members will be kept unknown to the other panel members until all rounds has been completed.
We used descriptive statistics as well as a random effects logit model with patient identity as a panel variable to assess associations.
While the average bias smoother lies on the respective identity lines in panels A/B indicating no evidence of average bias, panels C/D and E/F show evidence of nonlinear bias with the curvature of the smoother away from the respective identity lines.
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