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The PCR was carried out according to the following protocol: a denaturation step at 95°C for 2 min, 35 cycles of 95°C 30 sec, 55°C for 1 min, 72°C for 1 min followed by a final elongation step at 72°C for 5 min. Identity of representative RT-PCR products were confirmed by sequencing on an Applied Biosystems 3700 DNA Analyzer using dye-terminator chemistry.
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The identities of representative RT PCR products for each gene were confirmed by sequence analysis.
Species identity of a representative subset of the slugs collected in the third field screen and of the slugs used in the first experiment was characterized using an established DNA barcoding approach, based on DNA sequencing of a polymerase chain reaction (PCR -amplified fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) [ 23].
The taxonomic identities of the representative sequences from each OTU were classified via the Ribosomal Database Project (RDP) classifier with the SILVA databases (Wang et al. 2007).
Those representatives should protect the identity of the represented authors and performers for as long as that is possible.
Includes data on 18S rRNA gene read abundances, 16S rRNA gene read abundances, assigned taxonomic identity of OTUs and representative sequences.
Given the homeotic alterations observed during flower development of 35S TAGL1 plants, expression level of floral organ identity genes representative of A-, B- and C- class MADS-box genes were analyzed.
Her scholarship has addressed such issues as the relationship of citizens' trust in government to the racial identity of their elected representatives, the ways neighborhood conditions influence racial and political attitudes, the roots of competition and cooperation between minority groups, and the consequences of housing-mobility programs for political participation among the poor.
Indeed, in a properly structured system, there will be an "identity of interests" between representatives and the electorate (Mill 1992, 22).
Without localism and a sense of identity, what will be left of representative democracy?
Moreover, the alignment identity of the corresponding OTU representative to the Campylocentrum micranthum reference was only 92.4% (data not shown).
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