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This scattering indicates that some pairs of sequences can appear in close proximity according to identity distance between their catalytic sites, and have various similarity level based on LMS distances.
Interestingly, on the comparison between full-length LMS distances and identity distances, as well as the comparison between full-length LMS distances and identity distances, points are scattered in the region corresponding to low identity distance.
Here, we compare the distances derived from LMS with alignment-based distances: distance based on percentage identity between catalytic domains ( = identity distance), distance based on BLOSUM scores between catalytic domains ( = BLOSUM catalytic distance), and distance based on BLOSUM scores between full-length sequences ( = BLOSUM full distance).
Finally, we calculated an identity distance matrix for each one of these blocks.
We build a hierarchical tree using complete linkage (furthest neighbor) agglomerative clustering, based on pairwise identity distance (100%% − identity %).
We also drew phylogenic trees with an identity distance calculated from the proportion of conserved amino acids in pairs of repeats (see Additional File 8(B)).
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As expected, sequence alignment-based, i.e., identity distances and BLOSUM distances show very high correlation, with coefficients between 0.73 and 0.88.
Two genetic distances integrating different time steps were used, the Rozenfeld (RD; based on alleles divergence) and the Shared Allele (SAD; based on alleles identity) distances.
But the constant discussion of Islam has made Muslims feel more of a sense of identity and distance than before.
However, grouping at 92% identity (Levenshtein distance of 5) clearly groups outlier clades with the main group.
Pairwise sequence alignment, multiple sequence alignment, % identity calculation, distance (divergence) calculation, phylogenetic tree reconstruction and bootstrap analysis were performed using software Clustal×1.8.3 [44], [44].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com