Exact(1)
Each step of the pipeline (aligning probes to transcripts, parsing blast result, identifying transcript specific probe-clusters, creating transcript specific CDF file, processing using R, retrieving and displaying of differentially regulated or detected alternatively spliced forms) was checked manually to ensure that the software works as intended.
Similar(59)
These findings provide a resource for identifying transcripts that are substrates for adenosine methylation and reveal insights into the epigenetic regulation of the mammalian transcriptome.
Since these two types of probes cannot be easily identified, expression profiles obtained using these files might not be very useful for identifying transcript-specific expression status.
This illustrates the sensitivity of next generation sequencing technology in identifying transcripts with low expression.
This was achieved by identifying transcripts, which were mapped to genomic loci annotated as sRNA.
The existence of the new transcript variant was confirmed by reverse transcriptase-PCR, semi-nested PCR using primers designed for the newly identified transcript variant.
a Target gene is the identified transcript from the peach transcriptome (http://www.rosaceae.org/node/35).org/node/35
Newly identified transcript isoforms are potential targets for breeding novel cultivars with high salinity tolerance.
First, we identified transcript expression profiles shared by sexual and asexual oogenesis.
The analysis of these sequences requires computational tools to align the transcripts to the genome and identify transcript isoforms.
Both state and emission probabilites were calculated using ∼30 kb training set of manually identified transcript and non-transcript regions.
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