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Because there is not one marker that is specific to MPCs, all parameters must be taken into consideration when properly identifying this cell population.
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We identified this cell as the interneuron DVC by differential interference contrast (DIC) microscopy (Fig. 2C).
The H358 adenocarcinoma cell line harbours a K-Ras mutant and no EGFR mutations, yet our predictor and data of others [ 13] identify this cell line as sensitive to EGFR inhibition.
While phenotypic identification of these cells within tumors has been controversial, evidence exists supporting the importance of CD133, CD90, CD13, EpCAM and CD44 as potential markers to identify this cell population [ 14- 19, 24].
Whatever the genotype studied, in vitro-cultured brain microvascular pericytes exhibited their characteristic irregular morphology and were positive for the alpha-smooth muscle actin and nerve-glial antigen 2 (NG2), which are markers previously used to identify this cell type [ 19, 20].
All this is further complicated by the lack of a specific marker and validated methods to unequivocally identify this circulating cell subset and by the presence of platelet microparticles in a standard assay for putative EPCs (41).
We addressed this question by identifying the cell types within synovium that showed evidence of AHR activation.
In addition, CD34+ keratinocytes were found to express α6 integrin more intensely than CD34– cells (p<0.05), identifying this population as an α6 integrin bright subset.
Studying the mechanisms of cyclophosphamide-resistance, he identified that this cell line had unusually high levels of ALDH activity and that cyclophosphamide resistance could be reversed by inhibition of ALDH activity with disulfiram.
No molecular markers have yet been identified for this cell type.
Weak expression of both mRNAs and inactive protein forms of MMP-11 and -23 could also be identified in this cell line.
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