As the LightPath story illustrates, Sagentia's R&D process both identifies the viability of medical device technologies and helps in bringing such devices to market.
Intriguingly, YOR283w was identified as essential for the viability of the sec14 kes1 double mutant [35].
In conclusion, we have identified miRs that alter the viability of bladder cancer cells in vitro, and that seem to impact on the cisplatin sensitivity in vitro.
Therefore, we performed a pilot screen utilizing patient-derived tumor cells and hTERT-HMECs to identify compounds that selectively reduced the viability of cancer cells without causing general cytotoxicity against normal breast cells [see Additional file 1, Table S1].
As we are particularly interested in the nuclear functions of Pif1, we sought to identify genes whose deletion affected the viability of or reduced the growth rate of pif1 -m2 cells, which are deficient in the nuclear form of Pif1 (Schulz and Zakian 1994; Zhou et al. 2000).
Next-generation sequencing is also replacing microarrays in high-throughput RNA interference screens: one can perform genomewide screens to identify genes that interfere with the viability of cancer cells using pools of short hairpin RNAs, and the results can be deconvoluted using next-generation sequencing [ 32].
We then carried out an SL counter-screen with siRNA against the 52 survival kinases to identify kinases whose inhibition would affect the viability of HuH6 cells with oncogenic β-catenin activity, but not that of HuH6 cells with low β-catenin activity (sh-induced knock-down of β-catenin).
This proof-of-principle project demonstrates the viability of new strategies to identify genomic features that distinguish subtypes of cancer from an etiologic perspective.
Having identified miR-564 as a regulator of cell viability, migration and invasion in breast cancer, we aimed at identifying the targets of miR-564 mediating these observed effects.
