Using microarray analysis we measured the mRNA levels from each of these four cell lines specifically identifying the expression levels of GPCRs and over a hundred of GPCR-related gene products and report them here.
These numbers are not very different from the situation in the human brain, where (based on the normal total brain tissue in Human Body Map dataset), ADAR1 is ranked at ∼0.9 and ADAR2 ∼0.5 (numbers are approximate, as different exons behave differently, and identifying the expression levels of the different splice variants is not trivial).
Through such a way, NGSmirPlant will identify the expression level of each kind of small RNA by calculating the number of total tags it belongs to.
Here, we identify the expression level of different FK506-binding proteins (FKBPs), primarily FKBP12 and FKBP51, as the key determinants for rapamycin-mediated inhibition of mTORC2.
Among the TDP-43 affected miRNAs that we identified, the expression levels of 17 miRNAs have previously been associated with NSCLC patient survival.
Here, we identify the expression levels of FKBP12 and FKBP51 as key determinants in defining whether a cell line or tissue is responsive to mTORC2 inhibition by rapamycin.
The BioXpress database project collects RNA-seq data from several publicly available sources such as TCGA (http://cancergenome.nih.gov/), ICGC (12) and Gene Expression Atlas (30), and uses a standardized method to identify the expression levels of the genes.
Subsequently, a group of 13 miRNAs showing differential expression in CRC tumors was identified with the expression level of miR-31 being correlated with CRC tumor stage [ 22].
In tumours of both genotypes, E-cadherin and Cytokeratin-positive areas were identified, although the expression level and pattern varied significantly.
Comparison of the expression profiles between cardiomyocytes and macrophages and subsequent functional studies identified that the expression level of Unc93b1 is a pivotal switch for the distinct TLR9 responses by regulating subcellular localization of TLR9.
