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Understanding this new reprogramming process and identifying the endogenous signals should suggest more natural ways of engineering coronary bypass grafts and revascularizing the heart.
The analytical procedures and methodologies described in this study should be generally applicable for identifying the endogenous phosphorylation sites in other GPCRs, overcoming the limitations of conventional approaches such as sequence motif analysis and site-directed mutagenesis.
Green nuclear spots identifying the endogenous activated form of PKCζ were also visible in the nucleus.
However, there are examples where this is not the case, and difficulties in identifying the endogenous ligands for this family have limited our understanding of how these regulators function in vivo.
Future studies on the biochemistry and physiology of Cld-like proteins (that lack the distal arginine and have no or very poor chlorite degradation activity) as well as a better understanding of the phylogenetic and biochemical relationship between Clds and DyPs will contribute to identifying the endogenous substrate of these enzymes.
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In particular, we identify the endogenous qualification problem as the problem of properly accounting for highly distributed, and potentially conflicting, causal knowledge when reasoning about the effects of actions.
The potential value of GFP has also been recognized in orphan receptor research, where various GFP-tagged therapeutic proteins have been constructed in an attempt to identify the endogenous ligand(s).
We also use this approach to identify the endogenous GRKs that are responsible for the agonist-induced phosphorylation of epitope-tagged beta2- adrenergic receptors (beta2ARs) overexpressed in rabbit ventricular myocytes that are infected with a recombinant adenovirus.
To identify the endogenous LdUba5, polyclonal antibodies were raised against the recombinant LdUba5 protein.
This protocol was used in particular to identify the endogenous molecules involved in the conduction along the nerve fibres of excitation without action potentials.
Here we use an affinity isolation/mass spectrometry (AIMS) approach to identify the endogenous ligand that is bound to HNF4α in mammalian cells and in mouse liver.
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