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By identifying the concentrations of these markers, the bionanorobot can determine the type of cancer present.
A calibration curve, at an appropriate wavelength, was prepared for each substance to help identifying the concentrations.
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The effective concentration 50 (EC50) was determined by fitting a sigmoidal dose response curve to the data and identifying the concentration responsible for 50% ADCC activity (Graphpad Prism v6.0f).
The changes of absorptions at 554 nm were applied to identify the concentrations of RhB, using a Shimadzu UV-2450 UV–vispectrophotometerer.
We finally solved the equations with the drugs used simultaneously and identified the concentrations and at which the same level of inhibition was achieved.
Extensive kinetics and dose response analyses of H2O2 were performed to identify the concentrations of H2O2 with similar cytotoxic activity toward different cell lines derived from different origins.
To estimate synergy, we followed the same procedure employed in the conceptual model above, where we first identified the concentrations and of the EI and the other drug required for achieving a desired level of inhibition, x, when used alone.
After excluding unqualified urine samples according to the standard sampling and storage procedures (GBZ159-2004), 23 pairs of subjects were selected within the 45 pairs of included subjects to identify the concentrations of DHBMA.
We next identified the concentrations of flutamide and CI-1040 monotherapies, which resulted in a level of reduction in cell viability and apoptosis similar to that observed with each of the combination therapy conditions.
We quantify the dependence of growth retardation on drug concentration and identify the concentration threshold above which growth retardation is evident.
For this structure, nonlinear dynamic analysis permitted to identify the concentration of inelastic deformations demands on the braces along the frame height.
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