Data analysis disclosed large numbers of DEGs between NS, AKs and tumor samples and identified the activation of several known oncogenic pathways in SCC, some of which were also activated in AKs.
Here, we identified the activation of the eEF-2 kinase-mediated autophagy as a new mechanism responsible for the insensitivity of cancer cells to growth factor inhibition.
IPA Canonical Pathways Analysis was performed to identify the activation of pathways from a library of canonical pathways.
As we have identified the activation of AMPK in monocytes, this may contribute to reduce visceral adiposity.
For assessment of apoptosis, we identified the activation of caspase-3, which is the common final executioner caspase for the apoptotic pathways.
The lists of transcripts altered ≥ 2-fold at each time point were imported into IPA to identify the activation of tight junction signaling pathways.
Upstream Regulator Analysis in IPA was performed to identify the activation of upstream transcriptional regulators that could explain the observed gene expression changes.
Indeed, we identified the activation of ER stress signaling pathways that are affected at transcriptional, post-transcriptional and post-translational levels.
Gene expression studies have also identified the activation of pathways related to innate immune responses and monocyte recruitment during early progression of chronic glaucoma (Fan et al., 2010; Howell et al., 2012; Steele et al., 2006).
In 2012, Vathipadiekal and his colleagues have reported the gene expression profile in ovarian cancer stem cells by affymetrix microarray and identified the activation of Notch signaling pathway, as well as several other genes unique to ovarian CSCs [ 129].
