Finally, we identify phosphorylation of STAT3 as a novel pathway activated by PPARβ/δ and show that inhibition of STAT3 phosphorylation blocks disease development.
On the other hand, Molina et al. [28], in a study of phosphopeptides in human embryonic kidney cells, identified phosphorylation of, unequivocally, eEF1A1 at Tyr29 and Ser163.
To reconfirm the presence of potential MPK phosphorylation sites in OsAux/LAX1, we conducted in-silico prediction to identify phosphorylation of the OsAux/LAX1 protein using the kinasephos2.0 server [ 39].
Besides, we identified phosphorylation of 11 BdCDPKs and 4 BdCDKs, which may function in defense response and regulation of cell division and differentiation [ 57– 57].
A global phosphoproteomic study of a melanoma tumour identified phosphorylation of LRRK2 at Ser as one of 5600 phosphorylation sites catalogued on 2250 proteins, but this was not investigated further [ 21].
Because anti-MST3(275–393) mAbs fail to recognize FLAG MST3 FL) following calyculin A exposure, the phosphorylation site(s) probably lies between residues 275 and 393 and is distinct from the previously identified phosphorylation of MST3 on Thr of its activation loop [ 19].
Previous studies had identified phosphorylation of two tyrosine residues (Y653 and Y654) as the key step in the shift towards an activated form of FGFR1 (Furdui et al., 2006).
These correspond to 3,994 uniquely identified phosphorylations of 2,214 peptide sequences of 1,252 proteins (Additional file 1: Table S1), including several conserved protein kinases, discussed below.
This is a very thorough description of an algorithm for identifying phosphorylation sites of Protein Kinase A (PKA).
It succeeded in identifying phosphorylation motifs for 11 of the 87 kinases tested, suggesting these are kinases with strict phosphorylation site requirements [8].
