However, current software for aligning RNA-Seq data to a genome relies on known splice junctions and cannot identify novel ones.
We have examined the expression of several known genes and identified novel ones in the adult rat hippocampus after a mild, transient, hypovolemic and hypotensive, global ischemic stress.
Our study not only revealed recurrently reported cross-cancer similarities, but also identified novel ones.
One valid criticism of forward genetic screens is that, in addition to identifying novel genes, one can easily invest a great deal of time discovering a new allele, or even rediscovering a previously identified allele, of an already characterized gene.
Being reference-free, our method is capable of focusing on novel sequence markers, such as ones identifying novel bacterial strains.
∙ A vast variety of data are visualized all together on the same screen helping in having an overview on whole data set, in interpreting data and in identifying novel opportunities and needs for one's specific study.
One approach for identifying novel genes associated with physiological pathways is to identify genes whose expression changes with differences in experimental treatment or phenotype.
For the academic, translational sciences refers to the desire of confirming and validating novel concepts and identify new ones.
The object of our present GWAS was to identify novel risk loci, i.e., ones not previously reported in the literature (Ahmed et al. 2009; Easton et al. 2007; Gold et al. 2008; Hunter et al. 2007; Murabito et al. 2007; Stacey et al. 2007, 2008; Thomas et al. 2009; Zheng et al. 2009) and to replicate these findings in an independent cohort.
We have developed a method to identify novel sequences in individuals using One End Anchor (OEA) reads from paired-end sequencing where one end of the pair can be uniquely mapped to the reference genome (termed "anchor read") while the other cannot be mapped at all (termed "orphan read") [ 11].
