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Accurately identifying modifications in protein sequences is a critical first step for PTM profiling, and thus our approach may benefit routine proteomic analysis.
Identifying modifications is done by calculating mass differences between monoisotopic peak masses.
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However, in some cases when large amounts of material are available, a very low percentage of fragment ions may still bear the O-GlcNAc moiety and may be useful in identifying modification sites (as exemplified in Figure 1) [ 52, 53].
We also considered the changes in trade patterns from a trade margin and product sophistication perspective to identify modifications in Latin American countries' trade basket composition.
DNA methylation and histone modifications are the most identified modifications that selectively activate or inactivate genes that control cell growth, proliferation, and apoptosis.
A previous structure activity relationship identified modifications to the base moiety of adenosine (Ado) that result in substrate and inhibitor activity.
A pilot study is not a hypothesis-testing study, but a necessary initial step in exploring a novel intervention to inform its feasibility and identify modifications needed in the design of a larger ensuing hypothesis-testing study.
We identified modifications in the expression patterns of these genes that could account for developmental differences between P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.
Interim analysis will identify modifications to the topic guide.
The set of identified modifications was then analyzed together in Step III: analysis of combined modifications.
The MS/MS data were analyzed to identify modifications such as phosphorylated residues.
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