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The assays have relaxed substrate specificity and are designed to identify the subclass or sub-subclasses of enzymes (phosphatase, phosphodiesterase/nuclease, protease, esterase, dehydrogenase, and oxidase) to which the unknown protein belongs.
In order to identify the subclass of TGF- β-producing granulocytes, careful microscopical observation for haematoxylin eosin-staining sections was performed.
For example, it is important to determine the clinical benefit of drug regimens that reduce small LDL particle fractions versus those that reduce all fractions simultaneously, as well as to identify the subclass distribution profile produced by each lipid-lowering agent, so that an agent may be chosen to appropriately modify the lipid profile of the individual patient [ 14].
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A backward selection procedure identified the subclass of significant variables among the following: stage (IIB vs III), Breslow (0 2 vs 2.1 4>4), Clark (II III vs IV vs V), ulceration (No vs Yes), regression (No vs Yes), lymph node status (No vs Yes) primary tumour location, sex (male vs female), age (<52 vs 52).
The first two digits ("class" and "subclass") identify the transport mode and energy-coupling mechanism.
For each protein family, we examined the first two EC digits of the catalyzed reactions and identified the dominant EC subclass containing the largest number of reactions.
The ability to identify a subclass of children with a higher illness severity was further corroborated when the gene-expression-based subclassification strategy was applied to a separate validation cohort of children with septic shock [ 14].
The identification of ultrastable sites in the human methylome led us to identify a subclass of CpG islands characterized by a very stable pattern of methylation encompassing the island and flanking regions, established early in development and maintained through differentiation.
Submap is an unsupervised subclass mapping method that identifies the correspondence or commonality of subtypes found in multiple, independent data sets potentially generated on different platforms.
Here, we identify a subclass of endogenous retroviruses (ERVs) that engages innate immune signaling in these cells.
For mechanism design with independent values, we identify a subclass of Vickrey Clarke Groves (VCG) mechanisms that induce efficient ex ante investments even with externalities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com