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These may be ubiquitinated forms, but because of their low abundance we were not able to identify the modification by mass spectrometry.
To identify the modification site(s), the precursor ion corresponding to the modified protein (m/z 1415.9 Da, 10+ charge state) was isolated for top-down experiments using μESI-FTICR-MS with ECD (Figure 1B, upper panel).
To identify the modification of cerebral capillaries by senile plaque, we used the super-resolution Structured Illumination Microscope (Nikon N-SIM, Nikon).
To identify the modification site of Photo-probe, the sample, after UV exposure, was subjected to trypsin digestion followed by peptide mapping using LC−MS.
There are several important reasons for measuring comorbidity: to correct for confounding factors, to identify the modification of effects, to predict study outcomes or the natural history of the disease, and finally for statistical efficiency [ 1].
Query: 'Match modified proteins in Reactome & MS details in PRIDE' —after finding a matching or conflicting modified residue position, one can then inspect the peptides and the spectrum used to identify the modification.
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Additionally, we demonstrated the use of PCA to identify the modifications in the biomass structure after different types of pretreatments and conditions.
As a complementary tool to FT-IR spectroscopy, multivariate statistical techniques have been employed to identify the modifications in the FT-IR spectra and evaluate the difference between the different treatments.
To identify the modifications needed was performed a energy assessment with this steps: mass and energy balance calculation; design data conditions comparison; analysis of differences between the actual values and the design reference.
Here, we used this tool to identify the modifications most predictive of promoters, enhancers, and gene bodies.
Such models can then be analyzed and, with the development and application of appropriate optimization techniques, one could identify the modifications that need to be made to the organism in order to achieve the desired biotechnological goal.
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