In order to further identify the localization of C30F12.4 in germ cells and embryos, we isolated gonads and embryos and found that C30F12.4 was mainly located in the cytoplasm around the germ cells and everywhere in oocytes (Fig. 7C).
Because of the significant role of calcium signalling in plant responses to different environmental factors, calcium participation in LMF effects was investigated using a pyroantimonate method to identify the localization of free calcium ions.
With the use of fluorescently tagged versions of these markers or of antibodies to known markers, it becomes much simpler to identify the localization of a protein of interest.
We then docked the E. coli SecA (ecSecA-closed) [12], [13] and E. coli SecB X-ray crystal structures [14] into the EM density map to identify the localization of the molecules and domains in this complex (Figure 1D).
Confocal and electron microscopy approaches were used to identify the localization of proteins known to participate to eCB-mediated retrograde signaling in the striatum and the hippocampus [1], [25], [26], [27], [28], [29].
To identify the localization of the LDLR aggregate, we co-stained LDLR with organelle markers for the Golgi apparatus (Figure 5A and 5B), lysosomes (Figure 5C and 5D), endoplasmic reticulum (Figure 5E and 5F), and early endosomes (Figure 5G and 5H).
We have thus been able to precisely identify the localizations of damage and the local compaction mechanisms, and to relate them to the characteristic structural heterogeneities of the tested carbonate.
This paper introduces a new system of landmarks with a distinctive pattern design for identifying the localization and orientation of a moving theatrical stage.
PSORT II analysis (Prediction of Protein Sorting Signals and Localization Sites in Amino Acid Sequences, http://psort.hgc.jp/form2.html) was used for identifying the localization sites.
We used Vkg-GFP, a GFP protein-trap that identifies the localization of the endogenous Viking protein as a marker of the type IV collagen (Morin et al., 2001).
