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With increasing miRNA data, it is desirable to design methods to identify guide strand based on machine learning algorithms.
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Using high-throughput sequencing of mutagenized alleles, we identified guide sequences that correlate with sgRNA mutagenesis activity and used this information for the rational design of all possible sgRNAs targeting the Ciona transcriptome.
This Joint Data Citation Principles identifies guiding principles for the scholarly citation of data.
These were manually identified, guided whenever possible by ESTs available via BLAST searching [ 18].
By focusing on several of these mutations and screening through guide RNA variants with different mismatch combinations, they were able to identify specific guide RNAs that stimulated Cas9 activity towards the mutated gene sequences but left the normal counterpart untouched.
Similarly, Texas introduced the Texas Indigent Defense Commission (TIDC) in 2001 with the goal of developing standards for indigent defense practices, in addition to a research base to identify and guide the implementation of evidence based practices (Laurin 2015).
The Digital Makerspace will work to identify and guide pioneering solutions with a targeted focus and a global impact.
We utilized these guidelines to identify and guide ethical considerations in relation to research with SCCY in Kenya.
The outcomes of these meetings are disseminated to the scientific community to identify gaps, guide research priorities, and improve clinical practice.
Note that the priority value/s are decided first, and these will help to identify and guide the stated aims of the communication.
Ultrasound can also determine the presence and extent of an abscess pocket, and may be able to identify and guide the removal of foreign bodies.
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