Sentence examples for identify cell viability from inspiring English sources

MTT (3,[4,5-dimethylthiazol]-2-yl) assay and trypan blue staining were used to identify cell viability.

MTT assay and DAPI staining were used to identify cell viability and apoptosis, respectively, and demonstrated that EGCG significantly increased cell viability and protected the cells from apoptotic death.

The use of a confocal laser scan microscopy based technique enabled us to identify cell type and test cell viability in tissue slices maintained for at least 4 days in culture.

We identified active compounds (non-class 4) identified by the cell viability assay in parental HEK 293 cells [for a description of the cell viability assay, see Supplemental Material (doi 10.1289/ehp.1002952)], or compounds that reduced activity in the 530-nm readout of more than four antagonist-mode assays, as cytotoxic and excluded them from further analysis of the antagonist-mode data.

Kinases play a crucial role in the regulation of Wnt/β-catenin pathway (and in some cases can be targeted by chemicals inhibitors) [ 18]; therefore, we carried out an siRNA kinome-wide loss-of-function screen (Qiagen, 687 genes - Additional file 3: Table S2) in the HuH6 parental cell line, to identify kinases required for cell viability in cells with high β-catenin (Fig.  3a).

Subsequent quantitation of cell viability identified Lenti-693 as the shRNA that produced most significant suppression of cell proliferation in all three models (P <0.01).

Indeed, organ culture studies of rat intervertebral discs identified that TGFβ3 improved cell viability and matrix retention in vitro (Risbud et al., 2006).

The most effective combinations resulting in more than 50%% inhibition of ECRF24 cell viability identified in the second screen are provided in Fig.  2a.

The AZ is identified by loss of cell viability, altered nuclear morphology, DNA fragmentation, elevated levels of ROS, and elevated enzymatic activities and expression of PCD-associated genes.

Interestingly, the broad-range caspase inhibitors Z-VAD.fmk and Boc-D.fmk were shown to inhibit nuclear fragmentation associated with apoptosis induced by 9cRA and still had no effect on over-all cell viability, identified by mitochondrial activity.

To simultaneously identify epithelial cells and investigate cell viability following taxol treatment the two-color fluorescent viability assay consisting of SYTO®63 and PI was combined with the detection of Ep-CAM.