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To identify base pair differences in genomic regions, the polymerase chain reaction (PCR) products of these genes were sequenced as follows: 8,756 bp for SBE1, 10,287 bp for SBE3, 3,444 bp for GBSS1, 6,914 bp for SSS1, and 2,314 bp for SSS2A (Table S1).
This analysis was completed by both the HMM approach previously described by Geissmann [26] to identify GC-rich regions and RNasim comparative analysis [26] that included Wu-blast 2.0 pairwise comparisons of sequences for searching similarities, and QRNA [89] to identify base substitution patterns in pairwise alignments that could correspond to a conserved RNA secondary structure.
We did identify base changes in several candidate genes, indicating a low rate of EMS effects on these sequences.
The RS Modification and Motif Analysis protocol was used to identify base modifications and methyltransferase recognition motifs.
After sequencing the libraries to coverages of 60× or greater, we used SMRTAnalysis 1.4 to identify base modifications and enriched motifs.
Sequences were assembled and aligned using the Staden package [ 57] to identify base substitutions and other alterations from the predicted consensus sequence.
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"They identify based on their own truth and leaning".
My child should never be categorized, labeled, judged and forced to identify based on his deepest, most private internal wars.
To identify base-pairing regions, we probed the structures of GcvB, SroC, and their hybrid in vitro with lead II) and RNase T1 (Fig 5A).
The sequences obtained from each isolate were joined together to form a continuous whole gene sequence, aligned with ClustalW (http://www.ebi.ac.uk/clustalw/) and compared to the wildtype to identify base-pair mismatches.
Viral infections are difficult to identify based on PCT measurements.
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