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To identify alignment and amplification artifacts, custom R scripts were used to analyze the aligned reads of all single-end libraries with more than 10 duplicates (defined as having the same chromosome, start and strand values).
This method allowed us to identify alignment blocks with TFBS turnover (gain or loss) while controlling the FDR at 10%.
At the end, we curated the final list manually to identify alignment artefacts of the mapping algorithm.
RAPSearch, however, requires a substantial memory footprint to identify alignment seeds, due to its use of a suffix array data structure.
To identify alignment sections of random similarity prior to tree reconstructions, we used ALISCORE, which, compared to the commonly used Gblocks [ 81], is not dependent on the specification of an arbitrary threshold [ 65].
Given a multiple alignment of orthologous mRNA sequences, our goal is to identify alignment columns that are conserved beyond what would be expected by chance if the corresponding sites were evolving only under the selective pressure on the amino acid they contribute to encode.
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In both cases the BLAST output was parsed to identify alignments that had no mismatches in a match length of 18 bases or less and one mismatch in longer alignments.
A computer search of the Lifeseq Foundation database (Incyte, Cambridge, UK) was performed to identify alignments between the 20 known N-terminal amino acids of glycosylated PIF (Todorov et al, 1996) and translated Incyte sequences.
The first stage of MUMmer alignment involves identifying alignment anchors.
The identified alignment is not necessarily optimal if there is a gap between the score and the upper bound.
While the Viterbi decoding assigns gain only if the whole alignment is correctly predicted, posterior decoding assigns gain +1 for each correctly identified alignment column.
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