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The fold change between with and without mechanical strain treatment had to be at least 1.5 fold to identify a transcript as being altered (p<0.05) and these genes list was compiled and exported into DAVID (http://david.abcc.ncifcrf.gov/) for further analysis.
Tags mapped to a unique sequence are the most critical subset of the DGE libraries as they can explicitly identify a transcript.
Tags mapped to a unique sequence are the most critical subset in DGE libraries, as they can explicitly identify a transcript.
Tags mapped to a unique sequence are the most critical subset of the DGE libraries because they can unambiguously identify a transcript.
Importantly we did not identify a transcript that was differentially expressed only in D2 or B6 mice suggesting that there are no sequence polymorphisms in important transcription factor binding sites.
However, RT-PCR using various primer sets on an RNA panel that included total RNA from human brain, heart, kidney, liver, lung, testis, colon, small intestine, placenta and skeletal muscle failed to identify a transcript.
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We identified a transcript at ≥90% confidence for 6,238 (40%) of the 15,552 currently annotated human genes represented on the microarrays.
We had previously identified a transcript nearly identical to the OnubOR7a gene from male antennae of O. scapulalis (Fig. 3C); however, we had interpreted it as an intraspecific variation of a single locus.
The isoform signature uniquely identifies a transcript structure, and can therefore be used as a key in databases of alternatively spliced isoforms, or to compare alternative splicing predictions produced by different methods.
During those studies, we identified a transcript [GenBank accession no.
We also identified a transcript encoding a glutaminyl-peptide cyclotransferase (glutaminyl cyclase; GC).
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