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As a result, 9,596 unique sequence identifiers were assembled, which comprised 58.5% of the estimated cyprinid olfactory epithelium transcriptome.
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Reads from male, female and larval libraries were sorted by their unique multiplex identifiers sequences (MID) After quality evaluation, 457,445 of the obtained reads were assembled using MIRA [ 27].
The protein identifiers are displayed in additional file 4. Sequences were edited using chromas Lite 2.01 http://www.technelysium.com.au The edited sequences were assembled using BioEdit version 7.0.9.0 [ 34].
Images were assembled using Photoshop.
Chromatograms were assembled in DNASTAR Lasergene SeqMan Pro v.7.1.0.
Unigenes were assembled by BLAST similarity searches and annotated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology identifiers.
The KEGG metabolite identifiers were then assembled to find the corresponding KEGG reaction.
If the complete paths of the archetype nodes associated with each form component are stored instead of only the node identifiers, the structure of the EHR extract can be assembled without having to access the original archetype.
The locus list, including reference sequences from Cronobacter sakazakii ES15, was assembled using an in-house script which identified CDSs in Genbank accession CP003312.1 in which the < note > field included a COG identifier.
A non-redundant list of the databases was assembled and used to construct a presence-absence matrix consisting of rows of UniProt protein identifiers and columns of selected databases.
Smorgasbords were being assembled.
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