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The screen identified worms with mutations in a gene called obi-1.
When Hollopeter et al. looked for worms with genetic changes that can overcome the defects caused by a lack of FCHo, they identified worms with various mutations in the genes for the adaptor protein-2 complex.
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A newly identified worm-object is associated with an existing track only if the distance between the new centroid and that of the existing track is smaller than this threshold.
Contrarily, 34 (23.8%) mothers wrongly identified worm, breast milk allergy and brain problem as causes of colic.
The worm tracker automatically identifies worms and tracks their position as described previously [31]; position is defined as the worm's centroid (center of mass).
The tracker uses a size criterion to identify worms and because it is likely to be difficult, if not impossible, to establish size thresholds for mixed populations that reliably exclude both clumps of multiple worms and spurious objects, such as dust specks, that are smaller than single worms.
An infection with a great number of adult worms, known as a "heavy" infection, can have adverse effects on a dog's overall well-being, so identifying worms before the infection becomes "heavy" is the best approach.
Approximately 70% of worm genes are trans-spliced at their 5'ends, making it challenging to precisely identify worm promoter regions (Wormbook).
To identify worm proteins that interact with LET-756, we used the two-hybrid system in the MAV103 yeast with LET-756 fused to the Gal-4 DNA binding domain (pDB) as bait and two C. elegans libraries.
The assay was tested for specificity and optimized using genomic DNA extracted from identified male worms of Strongylus and cyathostomin species.
No homolog of TFIIIA has yet been identified in worms.
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