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Once the binding regions of AKIP 1A with PKAc and p65 had been identified, we next wanted to determine whether disruption of the complex played a functional role in cells.
After nonrandom structure at 2 different scales had been identified, we next sought to explore factors that could affect structure at these scales.
> -wrap-foot> Dare are means ± SEM for 12-week-old control (n = 6) and m-V59M (n = 5) mice Data were analysed using a Student's t test; no significant differences between control and mV59M mice were identified We next carried out stress tests on cardiac function.
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Since agonists for mouse TAAR3 5 have been identified [8], we next asked whether these agonists also activate intact orthologs of other mammals.
To identify DEGs, we next performed a pair-wise comparison between tumor and non-tumor tissue using a fold change cutoff of reads per kb per million (RPKM) values larger than 2 and an FDR-adjusted P value less than 0.01 [ 17].
In order to investigate the characteristics of the identified pathway structure, we next carried out a steady-state analysis for ERK and B-Raf responses under the constant inputs of Ras- and Rap1-GTPs (Figures 5B and 5C).
Using the set of potential DGRs identified by DiGReF, we next examined the corresponding RTs in further detail.
To identify the suppressor we next tested mutations in genes that are known to be located in this chromosomal interval.
To identify functional themes, we next clustered all of the stemness-on and –off modules detected by S-MAP according to their patterns across the expression compendium (Figure 5A).
Since SPPrl accumulates in the presence of SPPD/A and can be identified by Western blotting, we next used BN-PAGE to identify SPPrl/SPPD/A complexes.
As patients can move in and out of a state of REM over time, we next identified all connected (subsequent) visits in REM to identify sustained REM periods.
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