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To explore this possibility, for each MTB we identified, we computed 7 non-expression features, including the number of mRNAs and miRNAs in it, the density of 1's in the MTB, in the same rows, columns or either but outside the MTB, and the MTB type.
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Once the base layer is identified, we compute the aspect ratio (AR) of the cluster, defined as the ratio of the depth to the spread of the base layer (Fig. 2D).
Per AC method, the tumour was manually identified, and we computed the SUVmax in the tumour.
For each identified MRM, we computed the fraction of miRNA-mRNA pairs with expression correlation more negative than a threshold t.
As a validation of the identified modules, we computed whether aging-related genes stored by GenAge (de Magalhaes & Toussaint, 2004), a database providing a comprehensive overview of aging-related genes in humans and model systems, were enriched within modules A F (Fig. 2) [Data S1, Supporting information].
To identify pauses, we computed the dwell time of Pol II at each nucleotide position.
For all promoters with an identified TSS location, we computed the average occurrence of deletion events per sequence at each position relative to the TSS (see Methods).
In order to identify these fragments, we computed the distribution of reads in the contact map (see Additional file 1: Figure S2B).
To identify miRNA families, we computed all-against-all pairwise sequence alignments of miRNA 5′- and 3′-arms using USEARCH 6.0.307 (Edgar 2010).
To identify differentially expressed genes, we computed "smooth t-statistics".
To verify the coding capacity of ORFs identified in ERE1 and ERE2 we computed the ratio of non-synonymous to synonymous substitutions [ 16, 17] on the DNA sequence containing the ORF of interest.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com