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The platelet population in these whole blood samples was identified using flow cytometry.
Viable and necrotic cells were identified, using flow cytometry with forward and side scatter parameters.
As shown in Additional file 1: Figure S1, the RR and RU cells were readily identified using flow cytometry.
First, since putative CSCs were originally identified using flow cytometry we have assumed that this assay can be reasonably translated into an IHC based equivalent.
The T cell and NK population in the control and extract treated PBMC were identified using flow cytometry surface antigen immunophenotyping.
Apoptotic cells were identified using flow cytometry (Beckman Coulter Epics XL Flow) and the Annexin-V-PE apoptosis detection kit (BD Pharmingen, Franklin Lakes, NJ, USA) as described by the manufacturer's protocol.
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To assign SNP loci on the genetic map to chromosome arms, centromere positions were identified using flow-sorted chromosome arms following the method described in Simkova et al. [ 24]; results of this work will be described elsewhere (Bhat et al., in preparation).
More recent data from human studies [ 62] suggest that oxygen supply dependency may occur with as little as 30% reduction in gastrointestinal blood flow, with mucosal supply dependency (identified using continuous flow gastric tonometry) occurring before global splanchnic supply dependency can be identified (using portal venous CO2 measurement).
After two washes (PBS-albumin, 300 g, 10 min, 25°C), and fixation (2% paraformaldehyde in PBS, 30 min) the levels of phagocytosis were revealed by the florescent intensity of cell-engulfed beads, and could be identified using fluorescence flow cytometry (FACScalibur, BD Biosciences).
Only one study was identified using a flow chart to show the number of non-participating but eligible participants.
The tryptic digests of the proteins were separated and identified using a flow rate of 400 nL/min with an RP-nano-UPLC system (nanoACQUITY UPLC, Waters, Milford, MA, USA) coupled to an ion trap mass spectrometer (LTQ Orbitrap Discovery Hybrid FTMS, Thermo, San Jose, CA, USA) equipped with an electrospray ionization source.
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