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Three major damage mechanisms are identified: the matrix cyclic softening which overloads fibers leading to their progressive rupture; the interfacial degradation of these broken fibers and their oxidation by the environment.
A shotgun proteomic analysis of metastatic melanoma cell-line secretomes also identified the matrix proteins fibronectin and extracellular matrix protein 1, as well as the matricellular proteins SPARC (secreted protein, acidic, cysteine rich) and osteopontin [ 26].
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Identifying the matrix properties that permit directing stem cell fate is crucial for expanding desired cell lineages ex vivo for disease treatment.
This method does not derive an average, but identifies the matrix that is most representative of each group (these were used as the class matrices in Figure 2).
When the null hypothesis of incongruence is rejected, a posteriori tests should be used to identify the matrices that can be combined in a supermatrix, and those that should be analysed separately, or combined in a supernetwork approach [ 48, 54].
FluxAnalyzer identified the stoichiometric matrix and elementary modes.
They identified the extracellular matrix protein TGFBI as the most under-expressed gene in the paclitaxel-resistant cell line.
Two biochemically and morphologically differentiated entities have been identified: the interstitial matrix and the extracellular basement membranes (BMs).
Recent work has identified the Golgi matrix protein golgin-160 as a key determinant of dynein localization to the Golgi (Yadav et al., 2012).
HEEBO analysis identified the extracellular matrix-related pathways, but failed to identify significant enrichment in the Wnt signalling pathways, which are known to play a critical role in DTF [55] [60].
This method identifies the spatial matrices, namely mass matrix, damping matrix and stiffness matrix, which describe the properties of dynamic structures, using experimentally measured FRFs (Frequency Response Functions) within the frequency range of interest.
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