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These reference maps have now 255 identified spots, corresponding to 84 protein entries.
Preliminary work on facial expressions, by tracking the motion of 20 identified spots, has been done by Suma et al.[13].[13]
In our study, 7 out of 16 down-regulated spots in the group belonging to photosynthetic enzymes were identified (spots LD2, LD5, LD7, LD10, LD13, LD14, LD15 and LD16).
The lower bands might account for the identified spots on the 2D gel with lower molecular weight.
The assigned and identified spots are indicated with arrows and numbered in the Western blots and the corresponding silver-stained gel shown in Figures 2 and Figure 3.
The gels were CBB stained, matching protein spots were excised and 30 spots were successfully identified by MALDI-TOF MS. Of the identified spots, 19 represented known lumen proteins while the remainder were stroma proteins or proteins of unknown localisation.
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The protein abundance is presented as the percentage of the total spot volume associated with each identified spot.
Second identified spot was oxygen-evolving enhancer protein 1 (OEE33, gene name psbO).
In 2-D gel, three proteins were unambiguous identified (spot 33, 34 and 35, respectively).
They drew a layout identifying spots where there were slowdowns.
The Otsu algorithm [ 56] was used to identify spots.
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