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The protein abundance is presented as the percentage of the total spot volume associated with each identified spot.
In 2-D gel, three proteins were unambiguous identified (spot 33, 34 and 35, respectively).
Second identified spot was oxygen-evolving enhancer protein 1 (OEE33, gene name psbO).
Analysis was carried out in two overall steps: (i) localization of particles in micrographs and quantification of integrated fluorescence emission intensity from each identified spot and (ii) colocalization of detected particles in different color channels.
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These reference maps have now 255 identified spots, corresponding to 84 protein entries.
Preliminary work on facial expressions, by tracking the motion of 20 identified spots, has been done by Suma et al.[13].[13]
The lower bands might account for the identified spots on the 2D gel with lower molecular weight.
The assigned and identified spots are indicated with arrows and numbered in the Western blots and the corresponding silver-stained gel shown in Figures 2 and Figure 3.
The average spot volumes of identified spots ranged from 112 to 121 for the six samples.
We did not find typical phloem sap proteins, such as PP1 protein among the identified spots.
Whereas this fact was encouraging, the low percentage of identified spots requires further attention.
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