We identified separation-of-function mutations of Swi3, which leads us to propose that Swi3 utilizes different molecular mechanisms to regulate the replication checkpoint and sister chromatid cohesion.
We identified separation-of-function mutations within this domain that abolished rpo26 ∆ complementation without affecting tgs1 ∆ suppression.
Most theologians recognize the allegorical character of most of the traditional imagery of heaven, hell, and purgatory, and the church's catechism identifies separation from God as the greatest punishment of the "eternal fire" of hell.
Scores plots were examined to identify separation between adhesives composed of a primary polymer (PVAc or acrylic) and those where a secondary polymer (CN) is also present.
Partial least square discriminant analysis (PLS-DA), a supervised technique, identifies separation between groups and was performed in order to maximise separation between variables.
In this study, we identify separation-of-function mutations in the TEN-domain of hTERT.
As the purpose of the screen was to identify separation-of-function mutants, we did not further characterize many of the mutants from the screen.
If Bub1 and Mad1 have an additional role in the checkpoint, unrelated to Mad1 kinetochore localization, it should be possible to identify separation-of-function mutants that preserve kinetochore localization but are deficient in checkpoint signalling.
The purpose of supplement to Figure 1 is to show the raw data for a variety of hTERT mutants that were screened in order to identify separation-of-function mutants.
To identify separation-of-function hTERT mutants that do not interact with POT1-TPP1 but retain near wild-type telomerase activity in vitro, we carried out direct telomerase extension assays.
In this study, we identify separation-of-function mutations in the TEN-domain of human telomerase reverse transcriptase (hTERT) that disrupt the interaction of telomerase with TPP1 in vivo and in vitro but have very little effect on the catalytic activity of telomerase.
