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Previously, using different three-dimensional model systems, four distinct morphologies of breast cancer cell lines have been previously identified; Round, Mass, Grape-like, and Stellate [ 45].
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All LCOs identified rounded or "squiggly" inclusion bodies and displayed well-resolved emission spectra upon binding.
Polyclonal phage ELISAs on target and control antigens were used to identify rounds in which clones had been successfully enriched.
One statement identified in round 2 was missing in round three.
Four of the five important indicators were the same as those identified in Round 1, where fluctuations in heart rate in Round 2 replaced agitation in Round 1. Increased heart rate from baseline and reduced oxygen saturation in Round 2 replaced agitation and nasolabial furrow in Round 1 for useful pain indicators.
Of these, eight which were identified in round 1 appeared consistently through to round 3.
This is due to the fact that Round 1 encodes many irrelevant k-mers, whereas Round 2 encodes only the important k-mers identified in Round 1.
The draft questionnaire reviewed in Round 2 incorporated the themes and related questions identified in Round 1.
The aim of round 2 was to evaluate the level of consensus among the expert panelists on the leadership attributes identified from round 1, with a view to retain critical ideas for the next round.
The top five usefulness indicators rated in Round 2 were the same as the usefulness indicators identified in Round 1, with the exception of increased heart rate from baseline replacing agitation.
However, not all barriers identified in Round 1 were retained by the expert panelists as the iterative rounds progressed and as the expert panelists re-evaluated their ideas in relation to the group summaries and statistics.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com