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Furthermore, chromatin immunoprecipitation (ChIP) analysis has identified Responsive to desiccation/Cold-regulated (RD/COR) genes as downstream targets of RAP2.1 in vivo.
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The need to reveal biomarkers and understand the resistance will help to identify responsive versus non responsive patients to 17AAG.
In agreement with previous finding, we also noticed a significant correlation between stress-responsive genes and age-responsive genes: 21% of the whole body age-responsive probe sets identified in our study were also identified as responsive to paraquat, or H2O2 or tunicamycin in a previous work [ 30] (see Additional file 1 for multi cluster identification).
Furthermore, Oases produces isoforms from a locus; the longest transcripts of each locus were selected to construct a non-redundant set of contigs to identify responsive transcripts.
A G-test (FDR [False Discovery Rate] <0.01) of the RPKM-derived read counts was performed to detect differences in gene expression between control and –P-treated plants, and to identify responsive wheat transcripts under –P conditions (step 7 in Figure 1).
No standard pharmacogenetic tests exist to identify responsive genotypes.
Previous rodent visual fMRI studies used block-design stimulation paradigms to identify responsive regions [16], [25], [26].
For RT-qPCR, 20 μM DMBQ or 30 μM peonidin was applied twice to the same seedling plates at 2-day intervals and haustorium formation was scored 24 h after each time to identify responsive and non-responsive plants.
Untreated and metronomic cyclophosphamide-treated human U251 glioblastoma xenografts were analyzed on human microarrays at two treatment time points to identify responsive tumor cell-specific factors and their upstream regulators.
About two-third of the metabolites that we identified as responsive to drought and associated with tolerance were unknowns (Fig. 5).
Two groups display a nuclear Arc signal which can be clearly identified as responsive to shock-US delivery: the DS and LI groups (group main effects: F3,12 = 37.40, P<.001; P's<.01 for DS/LI vs. control, Tukey's HSD).
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