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The activation energy is estimated by means of both integral (Starink) and differential (Friedman) isoconversional methods, as a function of the extent of conversion, corresponding to the identified reaction intervals.
A new methodology of "discrimination of rival kinetics" was developed to obtain a set of accurate kinetics for the identified reaction mechanisms, and this approach allowed us to develop more robust reactor design by making full use of the experimental data, avoiding unnecessary laboratory experiments.
After some experimentation[ 16] we identified reaction conditions that allowed the target sulfonamide 2 to be isolated in good yield [Eq. (2), Scheme 1].
In very few cases, this did not coincide with the visually identified reaction time (point of largest curvature in the log[p]-t plot) and was corrected after visual inspection.
The bipartite metabolic networks were constructed based on the connection matrix of updated KEGG reactions database according to Stelzer and Zeng [ 97] with addition of newly identified reaction catalyzed by lactate oxidase (Lactate + O2 = > Pyruvate + H2O2) with provisional R numbers of R10001 (C00186 + C00007 = > C00022 + C00027) and R10002 (C00256 + C00007 = > C00022 + C00027).
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The bottleneck nature of several of the identified reactions was confirmed by earlier studies on over-expression of the identified target genes.
The model development workflow identified reactions that are in some cases unique to each reconstruction.
Supported by statistical evaluation, a target potential is attributed to the identified reactions in a quantitative manner.
Once identified, reactions identified as having unsatisfied reversibility can be marked if appropriate, as only-forward or only-backward based on the signs of their fluxes.
Moreover, the authors described the formation of α-ketovalerate and α-isoketovalerate as intermediates of the same pathway through not identified reactions.
The gel electrophoretic analysis of PCR products performed during array construction identified reactions that either had failed to produce a product or had produced more than one product.
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CEO of Professional Science Editing for Scientists @ prosciediting.com