Next, individual clones from one of the identified pools were analyzed by colony PCR for the respective transposon integration.
Following standard PCR of DNA pools of twelve cultivars, HRM analysis consistently identified pools with a SNP-containing cultivar.
Mosquitoes were collected fortnightly, identified, pooled in up to 50 specimens and tested using a One-Step SYBR Green-based Reverse Transcriptase-Real-Time PCR [ 16].
Fractions that contained complete BamABCDE complexes were identified, pooled and concentrated, and the concentration of the purified protein was determined as described above.
To start the program, Treasury will ask banks, like Citigroup or JPMorgan Chase, to identify pools of residential and commercial real estate loans that they will be willing to sell through an auction.
The Web site also lets users identify pools they can swim in; those featured on the site include pools at 1,200 clubs Speedo sponsors in the United States, as well as some 1,500 others.
To identify pools with positive signal, we first used an automated classical outlier detection method, that we called the "automated scoring" method.
In conclusion, the RBH approach provides a fast, efficient, and stringent although not exhaustive methodology to identify pools of orthologs between species when extensive proteomes are available.
Moreover, treatment of mouse cultured hippocampal neurons with detergents to eliminate the soluble pool of microtubules identified a pool of detergent resistant acetylated microtubules at the AIS, not present at the rest of the axon.
Deletion pool source identifies which pool of deletion strains the deleted strain came from.
