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Turbid plaques were identified, picked using a twenty-gauge needle, and placed in 1 mL of 1/2 LB in an incubating shaker overnight at 30°C.
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One usually plugged-in Republican consultant who didn't want to be identified picking a fight with the new president said a continuation of the turmoil would quickly call into question the basic competency of the new administration.
Step Two: Once a palette is identified, pick shades of each of your favorite colors within it.
These settings resulted in a 4.11% protein FDR, with 373 distinct proteins identified (picking up extra proteins from carryover, in some cases) that spanned 3266 distinct peptides and 232 351 identified spectra.
Last week the Observer identified pick-up points in London, Birmingham, Leeds and Manchester, where casual workers are routinely picked up by unlicensed and uninsured employers.
Uber had identified pick-ups as a key area it's working to improve, since avoiding miscommunication and confusion at that stage can result in much more efficient service overall.
Now, Kintz says, the company believes it can protect consumers by stripping personally identifiable information from trips, and by identifying pick-up and drop-off locations only by zip code.
The bus company entered into a public-private partnership with Skillman, which gave $100,000 to the project, to create interactive planning software that lets program leaders identify pick-up and drop-off locations for students they watch after school.
A revised England Six Nations squad has to be identified and picked within the next month, giving Jones a relatively narrow window in which to recruit and install new assistant coaches if he feels that changes are required.
Single colonies were identified and picked out to verify the deletion of the target gene by extracting the genomic DNA from the transformants and amplifying the fragments with expected size in PCR.
Fluorescent colonies were identified and picked under an epifluorescence microscope (Nikon SMZ1500).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com