General proteomic studies have not found ETD preference over CID peptide ion analysis [39], while the combined use of ETD and CID MS/MS have been shown to increase the total number of identified modified peptides [40], [41].
There are no other significant adducts observed except the dehydrated form of previously identified modified peptides (Table S2).
PTMClust consistently, with the exception of SIMS versus MODmap, is able to improve on the overlap of the identified modified peptides between the different algorithms.
Furthermore, the inhibitory activity of azithromycin was largely ablated by selective removal of glycan groups, and we identified modified macrolides with increased potency against merozoite invasion.
By combining the sequenced tags from all histone methylation ChIP-Seq data [ 16] and employing signal processing techniques, we comprehensively identified modified positioned nucleosomes in functionally important regions and revealed their distribution throughout the human genome.
For each labeling experiment, we selected the digests that yielded the highest amount of reliably identified modified amino acid residues (mass spectrometric data set available in the Supporting Information).
