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The first step identified linked markers by a two-point analysis with the two-point option.
Most of the identified linked regions were large in size due to the limited number of individuals of the families used.
Yet in these four teleosts, a second Nanos1 gene was identified, linked to Loxl3, Dok1, Np620159.2, Sema4f and Ttc31.
We identified linked changes in the expression of human tissue kallikreins (hKLKs), a family of serine-proteases that are drawing increased attention due to their association with various forms of cancer and other diseases [ 22- 29].
Subsequent fine mapping of the identified linked region is performed to focus the candidate region and reduce the number of putative candidate genes, and mutation detection is done to uncover the genetic cause of the disorder [ 2, 3].
As a large number of candidate markers were identified linked to the LanR1 gene in this study (Table 1), we randomly selected five candidate SNP markers for conversion into simple PCR-based markers.
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When mapping segmental duplications, a duplication block was identified linking LOC_Os03g21040 (chromosome 3) with LOC_Os07g48460 (chromosome 7).
New co-chaperones are also being identified linking the heat shock proteins to previously unknown functions.
Additionally, a member of the retinoblastoma family and p21 were identified, linking these factors to XY germ cell G1/G0 arrest.
The capacity to identify linked mutations could be important for understanding the persistence [20] and clinical impact of mutant variants.
We employed exon capture with BSA to identify linked scaffolds.
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